Musashi1‐CreERT2: A new cre line for conditional mutagenesis in neural stem cells
Identifieur interne : 001532 ( Main/Exploration ); précédent : 001531; suivant : 001533Musashi1‐CreERT2: A new cre line for conditional mutagenesis in neural stem cells
Auteurs : Haruna Takeda [Singapour, Japon] ; Hideto Koso [Singapour, Japon] ; Lino Tessarollo [États-Unis] ; Neal G. Copeland [Singapour, États-Unis] ; Nancy A. Jenkins [Singapour, États-Unis]Source :
- genesis [ 1526-954X ] ; 2013-02.
English descriptors
- Teeft :
- A_id, Adult neurogenesis, Arrow heads, Cadmus, Cancer research, Cassette, Creert2, Customer a_id, Embryo, Embryonic, Embryonic development, Exon, Homozygous mice, Immunohistochemical analysis, Intestine, J_id, Lacz, Lacz expression, Lacz1, Lacz1 cells, Lateral ventricle, Lineage, Manoharj, Manoharj date, Mouse, Msi1, Msi1 expression, Neurogenesis, Northern blot analysis, Nsc, Olfactory, Precursor cells, Progenitor, Progenitor cells, Recombination, Reporter strain, Retina, Shorter transcript, Sustentacular cells, Tamoxifen, Tamoxifen treatment, Testis, Yfp1, Yfp1 cells.
Abstract
The RNA‐binding protein Musashi1 (Msi1) is one of two mammalian homologues of DrosophilaMusashi, which is required for the asymmetric cell division of sensory organ precursor cells. In the mouse central nervous system (CNS), Msi1 is preferentially expressed in mitotically active progenitor cells in the ventricular zone (VZ) of the neural tube during embryonic development and in the subventricular zone (SVZ) of the postnatal brain. Previous studies showed that cells in the SVZ can contribute to long‐term neurogenesis in the olfactory bulb (OB), but it remains unclear whether Msi1‐expressing cells have self‐renewing potential and can contribute to neurogenesis in the adult. Here, we describe the generation of Msi1‐CreERT2 knock‐in mice and show by cell lineage tracing that Msi1‐CreERT2‐expressing cells mark neural stem cells (NSCs) in both the embryonic and adult brain. Msi1‐CreERT2 mice thus represent a new tool in our arsenal for genetically manipulating NSCs, which will be essential for understanding the molecular mechanisms underlying neural development. genesis, 51:128–134, 2013. © 2012 Wiley Periodicals, Inc.
Url:
DOI: 10.1002/dvg.22357
Affiliations:
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Msi1‐CreERT2 mice thus represent a new tool in our arsenal for genetically manipulating NSCs, which will be essential for understanding the molecular mechanisms underlying neural development. genesis, 51:128–134, 2013. © 2012 Wiley Periodicals, Inc.</div></front></TEI><affiliations><list><country><li>Japon</li><li>Singapour</li><li>États-Unis</li></country><region><li>Maryland</li><li>Région de Kantō</li><li>Texas</li></region><settlement><li>Tokyo</li></settlement><orgName><li>Université de Tokyo</li></orgName></list><tree><country name="Singapour"><noRegion><name sortKey="Takeda, Haruna" sort="Takeda, Haruna" uniqKey="Takeda H" first="Haruna" last="Takeda">Haruna Takeda</name></noRegion><name sortKey="Copeland, Neal G" sort="Copeland, Neal G" uniqKey="Copeland N" first="Neal G." last="Copeland">Neal G. Copeland</name><name sortKey="Jenkins, Nancy A" sort="Jenkins, Nancy A" uniqKey="Jenkins N" first="Nancy A." last="Jenkins">Nancy A. Jenkins</name><name sortKey="Koso, Hideto" sort="Koso, Hideto" uniqKey="Koso H" first="Hideto" last="Koso">Hideto Koso</name></country><country name="Japon"><region name="Région de Kantō"><name sortKey="Takeda, Haruna" sort="Takeda, Haruna" uniqKey="Takeda H" first="Haruna" last="Takeda">Haruna Takeda</name></region><name sortKey="Koso, Hideto" sort="Koso, Hideto" uniqKey="Koso H" first="Hideto" last="Koso">Hideto Koso</name></country><country name="États-Unis"><region name="Maryland"><name sortKey="Tessarollo, Lino" sort="Tessarollo, Lino" uniqKey="Tessarollo L" first="Lino" last="Tessarollo">Lino Tessarollo</name></region><name sortKey="Copeland, Neal G" sort="Copeland, Neal G" uniqKey="Copeland N" first="Neal G." last="Copeland">Neal G. Copeland</name><name sortKey="Jenkins, Nancy A" sort="Jenkins, Nancy A" uniqKey="Jenkins N" first="Nancy A." last="Jenkins">Nancy A. Jenkins</name><name sortKey="Jenkins, Nancy A" sort="Jenkins, Nancy A" uniqKey="Jenkins N" first="Nancy A." last="Jenkins">Nancy A. Jenkins</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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